![]() ![]() This course is approved for 1.5 contact hours of P.A.C.E. Background: Cryptococcal meningitis is the most common systemic fungal infection in HIV infected patients. The Centers for Disease Control and Prevention, Division of Laboratory Systems is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. Identify and resolve commonly encountered problems during routine microscopy procedures.Identify the steps and results obtained in the India ink procedure.Describe the potassium hydroxide (KOH) procedure and its uses.Outline the steps of preparing a wet mount and interpret results. ![]() Interpret the results seen in the bacterial cells, with the effects of the various reagents during the Gram stain procedure.Sequence the steps in the Gram stain procedure.Identify the types of reagents used in the Gram stain procedure. submitted for chemistry tests, microscopy (cell count, Gram stain, and India ink stain), cryptococcal antigen detection test, bacterial and fungal culture.Express the purpose of the Gram stain procedure. This is a negative staining, useful for demonstration of capsule of bacteria and yeasts.Therefore, the unstained cells are easily discernible against the colored background. The acidic stain, with its negatively charged chromogen, will not penetrate the cells because of the negative charge on the surface of bacteria. Outline the steps of preparing a smear. Negative staining requires the use of an acidic stain such as India ink or nigrosin.ObjectivesĪt the conclusion of this program, participants will be able to: This basic level course is designed for new or existing public health and clinical laboratory professionals, individuals with a science background who are entering or reentering the microbiology field, or individuals needing training on basic microscopy procedures. ![]() This eLearning course will introduce laboratory professionals to microscopy procedures for smear preparation, as well as preparing and interpreting the results of a Gram stain, wet mount, potassium hydroxide (KOH), and India Ink procedures. This course is designed to explore the processes, procedures, and techniques necessary for completing routine microscopic examinations of laboratory specimens. Laboratory professionals should have basic knowledge and understanding of routine microscopy procedures and techniques. ACCEPTABLE RESULTS: No clumping of ink particles (OK) Year: DATE BY INK BRAND RESULT DATE BY INK BRAND RESULT Document all unacceptable results on QC DEVIATION FORM. ![]()
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